Journal: Experimental and Therapeutic Medicine

Article Title: Scutellaria barbata D. Don inhibits growth and induces apoptosis by suppressing IL-6-inducible STAT3 pathway activation in human colorectal cancer cells

doi: 10.3892/etm.2015.2692

Figure Lengend Snippet: Effect of EESB on the HT-29 cell cycle. Cells were pretreated with various concentration of EESB for 1 h, followed by stimulation with 10 ng/ml IL-6 for 24 h. (A) Cells were stained with propidium iodide and analyzed by fluorescence-activated cell sorting. The proportion of DNA in the S phase was calculated using ModFit LT version 3.0 software. (B) Quantification of fluorescence-activated cell sorting analysis. The data shown are averages with standard deviation from 3 independent experiments. #P<0.05 vs. cells treated with IL-6 but not EESB. EESB, ethanol extract of Scutellaria barbata D. Don; IL-6, interleukin-6.

Article Snippet: The HT-29 cell cycle progression was determined through flow cytometric analysis using a propidium iodide (PI) staining cell cycle assay kit (BD Biosciences, Franklin Lakes, NJ, USA).

Techniques: Concentration Assay, Staining, Fluorescence, FACS, Software, Standard Deviation

Journal: Experimental and Therapeutic Medicine

Article Title: Scutellaria barbata D. Don inhibits growth and induces apoptosis by suppressing IL-6-inducible STAT3 pathway activation in human colorectal cancer cells

doi: 10.3892/etm.2015.2692

Figure Lengend Snippet: Effect of EESB on HT-29 cell apoptosis. Cells were pretreated with various concentration of EESB for 1 h, followed by stimulation with 10 ng/ml IL-6 for 24 h. (A) Cells were collected and stained with Annexin V/PI, followed by fluorescence-activated cell sorting analysis. Double-negative stained cells indicate the live cell population; Annexin V-positive/PI-negative stained cells and Annexin V/PI double-positive stained cells represent early and late apoptosis, respectively; Annexin V-negative and PI-positive stained cells show dead cells. (B) Quantification of fluorescence-activated cell sorting analysis. The data shown are averages with standard deviation from 3 independent experiments. #P<0.05 vs. cells treated with IL-6 but not EESB. EESB, ethanol extract of Scutellaria barbata D. Don; IL-6, interleukin-6; UL, upper left; UR, upper right; LR, lower right; LL, lower left; PI, propidium iodide; FITC, fluorescein isothiocyanate.

Article Snippet: The HT-29 cell cycle progression was determined through flow cytometric analysis using a propidium iodide (PI) staining cell cycle assay kit (BD Biosciences, Franklin Lakes, NJ, USA).

Techniques: Concentration Assay, Staining, Fluorescence, FACS, Standard Deviation

Journal: Molecular Medicine Reports

Article Title: HOXB7-S3 inhibits the proliferation and invasion of MCF-7 human breast cancer cells

doi: 10.3892/mmr.2015.4009

Figure Lengend Snippet: Apoptotic rate following transfection for 48 h in MCF-7 cells. Con-B group, blank control group; Sn group, negative control siRNA; S3 group, S3 transfection group. The apoptotic rate in S3 groups was the highest compared with that in Con-B groups and Sn groups ( ** P<0.01). (A) Early apoptotic cells are defined as Annexin V + /PI − , whereas late apoptotic cells are Annexin V + /PI + . Data are representative results for one of the three replicates. (B) Percentage of Annexin V + /PI − MCF-7 cells following transfection with S3 for 48 h. HOXB7-S3 effectively enhanced MCF-7 cell apoptosis. Data are the average of three independent experiments each containing three replicates. HOXB7, homeobox B7; PI, propidium iodide; siRNA, small interfering RNA.

Article Snippet: The mouse polyclonal anti-human GAPDH antibody was obtained from Tianjin Sungene Biotech Co., Ltd. (Tianjin, China), siRNA was obtained from Shanghai Genepharma Co., Ltd., (Shanghai, China), the Cell Counting kit-8 (CCK-8) was purchased from Beyotime Institute of Biotechnology (Wuhan, China), Transwell plates were obtained from Corning Inc. (Corning, NY, USA), Matrigel was purchased from BD Biosciences (San Jose, CA, USA) and the Annexin-V-EGFP/propidium iodide (PI) Apoptosis Detection kit was obtained from EnoGene Biotech Co., Ltd. (Nanjing, China).

Techniques: Transfection, Control, Negative Control, Small Interfering RNA